RP-HPLC Method Development and Validation for Determination of Tigecycline in Bulk and Pharmaceutical Dosage form
نویسندگان
چکیده
Aims: To develop and validate a new, simple, rapid, precise accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the quantitative determination of Tigecycline in bulk pharmaceutical dosage form.
 Study Design:
 Place Duration Study: RBVRR women's college pharmacy, Barkatpura, Hyderabad, between june 2019 july 2020.
 Methodology: The RP-HPLC was developed on Sunsil C18 150 mm x 4.6mm 5µ column using acetonitrile : water (pH maintained at 3.5 with acetic acid) [70:30] as mobile phase flow rate 0.8 ml/min UV detection 250 nm.
 Results: exhibited linearity over concentration range 5-40 µg/mL (R2 > 0.999). analytical showed good precision % RSD below 2. suitable accuracy robustness.
 Conclusion: Validation done per International Conference Harmonization (ICH) Q2R1 guidelines.
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ژورنال
عنوان ژورنال: Journal of pharmaceutical research international
سال: 2021
ISSN: ['2456-9119']
DOI: https://doi.org/10.9734/jpri/2021/v33i39b32204